GE 17-0090-02 Cytiva Sephadex LH20 - Liquid Chromatography Media, 500g

Cytiva Sephadex LH-20 is a liquid chromatography media engineered for the fractionation of small biomolecules in organic and mixed aqueous-organic solvent systems. The media consists of hydroxypropylated dextran beads that have been crosslinked to create a stable polysaccharide network. This hydroxypropylation process is what gives LH-20 its defining characteristic: a matrix that is simultaneously hydrophilic and lipophilic. This dual nature enables separation mechanisms that go beyond simple size exclusion, providing chromatographic selectivity based on both partition and adsorption effects. As a result, Sephadex LH-20 can resolve closely related molecular species that would co-elute on conventional gel filtration columns.

In practice, Sephadex LH-20 is widely used for fractionating lipids, hormones, vitamins, steroids, fatty acids, and other low-molecular-weight compounds. It is a staple in natural product isolation, pharmaceutical analysis, and biochemical research laboratories. The media is compatible with polar organic solvents such as methanol, ethanol, and chloroform-methanol systems, as well as aqueous solvent mixtures, giving researchers flexibility in designing separation protocols tailored to their specific analytes. This 500-gram package is suitable for packing multiple analytical columns or a single preparative column, and the media scales effectively from bench-top research to industrial production environments where consistent, reproducible separations are essential.